BIOT 5132 - Advanced Methods in Biotech II

Credit Hours: 3 Lecture: 1 Lab: 5

Core Biotech lecture/lab that will focus on describing latest techniques of molecular biology and proteomics, including chromatographic separations of proteins, His-tagged protein an Ni-column purification, design and analysis of dual expression plasmids, RTPCR, 2-D gel electrophoresis and mass spec analysis of proteins, yeast two-hybrid assay.